The data were processed using the software Chem Station (ver.
Quantitative determination on HPLC Agilent 1200 with MS 6120 detector, Agilent Technologies, USA was performed.
Further, the solution was stirred for 15 minutes then the resulting solution was filtered through a filter with a pore diameter of 9 microns.Ĭonditions of chromatography. Solution of the dry extract prepared by dissolving an accurate sample of the extract (41.0 mg.) in 1.5 ml. Further, by diluting the obtained 200 mg/ml solution concentrations of 80 mcg/ml, 40 mcg/ml, 20 mcg/ml, 8 mcg/ml were prepared. The concentration of the resulting solutions was 200 mcg/ml. Were shaken until dissolved and diluted to volume with the same solvent. (accurately weighed) of standard samples (Cromodex, USA) in a volumetric flask of 25 ml. Standard solutions of eleutherosides were prepared by dissolving 5 mg. Thepurpose of the study is the development of method of quantitative determination eleutherosides B and E in the dry extract of Siberian Ginseng and its validation. They belong to different classes of biologically active compounds: eleutheroside A - a steroid eleutheroside B - a derivative phenyl-acrylic acid eleutheroside B1 - isofraxidin-7-glucoside eleuthero-sides D and E - lignans. The composition of the dry extract of Eleutherococcus identified several eleutherosides different structure - A, B, B^ C, D, E, F. Due to the presence of eleutherosides in the extract, Siberian Ginseng increases physical and mental work capacity, resistance to adverse environmental factors, improves metabolism, shows a small stimulatory gonadotropic and hypoglycemic effect. Liquid extract of Siberian Ginseng is used in physical and mental fatigue, neurasthenia and psychasthenia, functional exhaustion of the nervous system which is associated with reduced capacity for work, in vegetative neurosis and conditions after surgery.
Keywords: eleutherosides, chromatography, quantitative determination, validation method. Validation method is presented by the following characteristics: specificity, linearity and limit of quantification. Chromatography method in parallel with the standard samples eleutherosides B and E was performed. Quantification of eleutherosides B and E by HPLC were determined. In connection with the above, it is important to develop and validate a method for determining eleutherosides B and E. The dry extract of Siberian Ginseng contains eleutherosides B (syringin) and E (syringaresinol-4',4-O-bis-ß-D-glucoside) as the most pharmacologically active compounds. Postgraduate student, Laboratory of bioactive compounds Institute of Pharmacy E-mail: Pavlova Ludmila Anatolievna E-mail: Smirnov Valeriy Valerievich, Department of pharmaceutical and toxicological chemistryĮ-mail: method for determination of eleutherosides E and B in dry extract of Siberian GinsengĪbstract: The article presents a modern method for quantitative determination of eleutherosides B and E in dry extract of Siberian Ginseng (Eleutherococcus senticosus (Rupr. HPLC method for determination of eleutherosides E and B in dry extract of Siberian Ginsengīobok Maxim Nikolaevich, First Moscow State Medical University n.
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